zikv ns1 protein  (Sino Biological)

Journal: Scientific Reports

Article Title: Brain-infiltrating CD8 T cells retain functional activity to protect against acute Zika virus infection

doi: 10.1038/s41598-026-35079-3

Figure Lengend Snippet: ZIKV infection is associated with brain pathology-related responses in both juvenile and mature adult Ifnar1 − / − mice. ( A – D ) Four-week-old Ifnar1 − / − mice were infected intraperitoneally (i.p.) with the ZIKV PRVABC59 strain. ( A ) Schematic representation of the experimental strategy for ZIKV infection in juvenile mice. ( B ) Daily body weight changes during ZIKV infection. Error bars indicate SEM. ( C ) qRT-PCR analysis of ZIKV RNA levels in the brain at 5 days post-infection. ( D ) qRT-PCR analysis of neuronal injury-related and antiviral immune responses in the brain at 5 days post-infection. Data are representative of more than two independent experiments. ( E – H ) Five-month-old Ifnar1 − / − mice were infected with the ZIKV PRVABC59 strain. ( E ) Schematic representation of the experimental strategy for ZIKV infection in mature adult mice. ( F ) Daily body weight changes during ZIKV infection. Error bars indicate SEM. ( G ) qRT-PCR analysis of ZIKV RNA levels in the brain at 6 days post-infection. All juvenile and adult brain samples were analyzed on the same qRT-PCR plate using a shared standard curve. Mock control values (~ 100 copies) are displayed to provide a visual baseline for viral RNA levels. ( H ) qRT-PCR analysis of genes associated with neuronal injury and antiviral immune responses in the brain at 6 days post-infection. Data are representative of two independent experiments. All data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.005; **** P < 0.001 (two-tailed two-sample unequal variance Student t test).

Article Snippet: Two ZIKV strains, PRVABC59 (VR-1843) and MR766 (VR-84) were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Infection, Quantitative RT-PCR, Control, Two Tailed Test

Journal: Scientific Reports

Article Title: Brain-infiltrating CD8 T cells retain functional activity to protect against acute Zika virus infection

doi: 10.1038/s41598-026-35079-3

Figure Lengend Snippet: Reduced brain-infiltrating CD8 + T cells are associated with exacerbated ZIKV-induced brain pathology. ( A – H ) Five-month-old Ifnar1 − / − mice were infected with the ZIKV PRVABC59 strain. To block T cell egress into circulation, FTY720 was administered on days 0, 1, 4, and 5 post-infection. At six days post-infection, spleen and brain tissues were harvested for analysis. ( A ) Schematic illustration of the experimental mouse model. ( B ) Representative FACS plots (left) and bar graphs (right) showing the absolute numbers of T N , T CM , and T EM CD8 + T cells in the spleen of ZIKV-infected mice with or without FTY720 treatment. ( C ) Representative FACS plots (left) and bar graphs (right) depicting the absolute numbers of CD49d + PD-1 + CD8 + T cells in the spleen of ZIKV-infected mice. ( D ) Frequency of CD8 + T cells within the brain lymphocyte gate (CD45 hi CD11b − ). ( E ) Representative FACS plots showing the distribution of T N , T CM , and T EM subsets in the brain of ZIKV-infected mice. ( F ) Representative FACS plots of CD49d + PD-1 + CD8 + T cells in the brain. ( G ) qRT-PCR analysis of ZIKV RNA levels in the brain at six days post-infection. ( H ) qRT-PCR analysis of genes associated with neuronal injury and antiviral immune responses in the brain at six days post-infection. Data are representative of two independent experiments. All data are presented as mean ± SEM. * P < 0.05; ** P < 0.01; *** P < 0.005 (two-tailed two-sample unequal variance Student t test).

Article Snippet: Two ZIKV strains, PRVABC59 (VR-1843) and MR766 (VR-84) were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Infection, Blocking Assay, Quantitative RT-PCR, Two Tailed Test

Journal: Scientific Reports

Article Title: Brain-infiltrating CD8 T cells retain functional activity to protect against acute Zika virus infection

doi: 10.1038/s41598-026-35079-3

Figure Lengend Snippet: PD-1 blockade aggravates ZIKV pathogenesis in Ifnar1 − / − mice. ( A – C ) Four-week-old Ifnar1 − / − mice were infected with the ZIKV PRVABC59 strain. To assess the effects of PD-1 blockade, α-PD-1 antibody was administered on days 1, 4, and 5 post-infection. ( A ) Schematic illustration of the experimental mouse model. ( B ) Daily body weight changes during ZIKV infection. Error bars indicate SEM. ( C ) Survival rates of mock, ZIKV-infected, and ZIKV-infected mice treated with α-PD-1 antibody. The survival curves differed significantly among the three groups (log-rank [Mantel–Cox] test: χ² = 13.05, df = 2, p = 0.0015). Data are representative of two independent experiments. * P < 0.05; ** P < 0.01 (two-tailed two-sample unequal variance Student t test).

Article Snippet: Two ZIKV strains, PRVABC59 (VR-1843) and MR766 (VR-84) were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Infection, Two Tailed Test